Table 2.
Genome sequence-based characterization of AFP-positive strains
| AFP-positive strain | Phylogroup | Serotype | Sequence type | Plasmid replicon sequences | EAEC-associated genes | Antibiotic resistance genes |
|---|---|---|---|---|---|---|
| 2–005-03_S4_C1 | A | ONT:H10 | 43 | IncB/O/K/Z, IncFII, IncQ1 | aatA, aap | sul1, sul2, aph(3”)-Ib, aph(6)-Id, tet(A), dfrA14, dfrA7, blaTEM-1B |
| 2–011-08_S3_C1 | A | ONT:H10 | 10 | Col, IncFII | aatA, aap, astA | sul2, dfrA8, aph(3”)-Ib, aph(6)-Id, blaTEM-1B |
| 2–011-08_S3_C2 | A | O111:H12 | 43 | ColpVC, IncB/O/K/Z, IncFII, IncQ1 | aatA, aap | blaTEM-1B, tet(A), dfrA14, dfrA7, aph(3”)-Ib, aph(6)-Id, sul1, sul2 |
| 2–011-08_S3_C3 | A | ONT:H10 | 10 | IncFII | aatA, aap, astA | dfrA8, aph(6)-Id, blaTEM-1B |
| 2–316-03_S4_C2 | A | ONT:H10 | 10 | IncFII | aatA, aap | blaTEM-1B, sul2, dfrA8, aph(3”)-Ib, aph(6)-Id |
| 2–460-02_S3_C1 | A | O111:H12 | 3281 | IncB/O/K/Z, IncFII | aatA, aap | dfrA14, aph(3”)-Ib, aph(6)-Id, sul2, tet(A) |
| 2–460-02_S3_C2 | A | O111:H12 | 3281 | IncB/O/K/Z, IncFII | aatA, aap | dfrA14, aph(3”)-Ib, aph(6)-Id, sul2, tet(A) |
| 2–474-04_S3_C1 | A | ONT:H10 | 43 | IncB/O/K/Z, IncFII, IncQ1 | aatA | blaTEM-1B, tet(A), dfrA14, dfrA7, sul1, sul2, aph(3”)-Ib, aph(6)-Id |
| 2–474-04_S3_C2 | A | ONT:H10 | 43 | IncB/O/K/Z, IncFII, IncQ1 | aatA, aap | blaTEM-1B, tet(A), dfrA14, dfrA7, sul1, sul2, aph(3”)-Ib, aph(6)-Id |
| 2–474-04_S3_C3 | A | ONT:H10 | 43 | IncB/O/K/Z, IncFII, IncQ1 | aatA, aap | blaTEM-1B, tet(A), dfrA14, dfrA7, sul1, sul2, aph(3”)-Ib, aph(6)-Id |
| 3–073-06_S3_C2 | A | ONT:H10 | 43 | IncFII, IncQ1 | aatA, aap | aph(3”)-Ib, aph(6)-Id, tet(A), sul1, sul2, blaTEM-1B, dfrA7 |
| 7–233-03_S3_C1 | A | ONT:H10 | 10 | IncFII | aap, astA | - |
| 7–233-03_S3_C3 | A | ONT:H10 | 10 | Col, IncFII | aap | - |
| 12–05829 | B1 | O23:H8 | 26 | IncB/O/K/Z, IncFIB, IncFIC | aatA, aap, astA | - |
| 381–3 | A | O126:H2 | 10 | IncB/O/K/Z | aatA, aap | blaCTX-M-15, blaTEM-1B |
| 401,368 | A | O151:H12 | 10 | Col, IncFIC, IncI1 | aatA, aap, pic, astA | dfrA14, aph(3”)-Ib, aph(6)-Id, blaTEM, sul2 |
| AM22-15AC | A | ONT:H30 | 3075 | ColpVC, IncFII, IncY | aatA, aap, pet, astA | tet(A) |
| AM34-8 | A | O10:H32 | 1286 | IncFII, IncX1 | aatA, aap, pet | blaTEM-1B, qnrS1, tet(A), sul3, aph(6)-Id |
| CS01 | A | ONT:H30 | ? | IncB/O/K/Z, IncFIA, IncFIB, IncFII | aatA, aap | dfrA5, tet(B), sul1 |
| DEC6C | A | O111:H12 | 10 | IncFII | aatA, aap | - |
| ECM-1 | A | ONT:H30 | 2349 | IncFIB, IncFII, IncI1, IncX3 | aatA, aap | sul2, tet(A), aadA1, dfrA1, qnrB7, qnrS1, qnrS2, blaCTX-M-15, blaTEM |
| MRE600 | A | O150:H9 | ? | IncFII | aatA | - |
| NCTC9035 | A | O35:H10 | 10 | IncFIC | aatA, aap, pet, astA | - |
| NCTC9062 | A | O62:H30 | 34 | IncB/O/K/Z, IncFII | aatA, aap | - |
| NCTC9097 | B1 | O97:H- | 5466 | IncFII | aatA, aap | - |
| UPEC-46 | A | O116:H12 | 10 | IncB/O/K/Z, IncFII | aatA, aap, pet, astA | aadA1, sul2, tet(B), drfA1 |
EAEC genes searched: aggR (global virulence regulator), aatA (anti-aggregation protein transporter), aap (dispersin), pet (plasmid-encoded toxin), pic (protein involved in colonization) and astA (EAEC heat-stable enterotoxin 1); NT, non-typable.