Table 1.

Detection limit of the strip and the IDVET ELISA kit determined by ASFV-positive standard serum

Dilution	ELISA			Strip
	OD 450	S/P	Result	G/D × A-ROD × pixel	G/peak-ROD × pixel	Result
1:100	3.06	1.62	+	1035.91	0.79	+
1:200	2.83	1.49	+	984.93	0.68	+
1:400	2.74	1.43	+	866.30	0.60	+
1:800	2.53	1.32	+	794.95	0.51	+
1:1600	2.04	1.14	+	669.30	0.41	+
1:3200	1.34	0.66	+	646.25	0.38	+
1:6400	0.85	0.42	+	554.74	0.33	+
1:12,800	0.49	0.18	−	385.62	0.24	+
1:25,600	0.29	0.07	−	229.56	0.15	+
1:51,200	0.17	0.01	−	138.04	0.09	+
1:102,400	0.12	− 0.02	−	87.98	0.06	+
1:204,800	0.09	− 0.04	−	57.32	0.04	+
1:409,600	0.07	− 0.06	−	22.45	0.02	+
1:819,200	0.06	− 0.06	−	8.67	0.01	−
N	0.16	0.00	−	8.42	0.00	−

 − negative; + positive, S/P > 0.4 is considered positive. G/D × A means density value of the sampled outline, multiplied by its area. G/peak indicates graph peak density, maximum density value of sampled line points. ROD means relative optical density, inverse of gray level value with a logarithmic transformation G/D × A-ROD × pixel > 10 is considered positive