FIGURE 7.

scRNA‐seq analysis of tumor cell‐microenvironment crosstalk in HNSCC tumors after Mettl1 knockout. (A) UMAP of myeloid subsets, including neutrophils, Cx3cr1⁺ macrophages, Mrc1⁺ macrophages, Macro‐3, dendritic cells, Langerhans cells, and migrating cells, in Mettl1^cKO‐Ctrl and Mettl1^cKO mice. (B) Bar plots of the proportions of cell types and total cell number in Mettl1^cKO‐Ctrl and Mettl1^cKO mice. (C) UMAP of myeloid subsets of each sample. (D) UMAP of TIL subsets, including memory CD4 T cells, naïve CD4⁺ T cells, exhausted CD4⁺ T cells, naïve CD8⁺ T cells, NKT cells, Th17 cells, and Tregs, in Mettl1^cKO‐Ctrl and Mettl1^cKO mice. (E) Bar plots of the proportions of cell types and total cell number in Mettl1^cKO‐Ctrl and Mettl1^cKO mice. (F) UMAP of TIL subsets of each sample. (G) Circos plot showing significant crosstalk alterations between stromal cells and epithelial cell subclusters (Mettl1^cKO vs. Mettl1^cKO‐Ctrl). Abbreviations: Mettl1: Methyltransferase‐like 1; KO: knockout; TIL: tumor‐infiltrating lymphocytes; Mettl1^cKO‐Ctrl: K14CreER;Mettl1^wt/wt mice; Mettl1^cKO: K14CreER;Mettl1^fl/fl mice; Macro‐3: macrophage‐3; DCs: dendritic cells; UMAP: Uniform manifold approximation and projection