TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| S. enterica strains | ||
| DM17239 | ptsJ617::Km | This study |
| DM17238 | pdxK682::Cm | This study |
| DM17168 | ptsJ617::Km pdxK682::Cm | This study |
| DM17274 | purG3111 purE3043 stm4068-6::Tn10(d)Tc ptsJ617::Km | This study |
| DM16666 | Wild-type/pCV1 | This study |
| DM17210 | Wild-type/pDM1643 | This study |
| DM17242 | ptsJ617::Km/pCV1 | This study |
| DM17243 | ptsJ617::Km/pDM1647 | This study |
| DM17253 | ptsJ617::Km pdxK682::Cm/pCV1 | This study |
| DM17244 | ptsJ617::Km/pBAD33-SD1 | This study |
| DM17246 | pdxJ664 pdxH673 ptsJ617::Km/pDM1594 | This study |
| Plasmids | ||
| pBAD33-SD1 | Modified pBAD33 (Cmr) | 55 |
| pCV1 | Modified pBAD24 (Amr) | 50 |
| pDM1594 | pBAD33-SD1 expressing S. cerevisiae SNZ3 (Cmr) | 55 |
| pDM1643 | pCV1 expressing S. enterica pdxK (Amr) | This study |
| pDM1647 | pCV1 expressing S. enterica ptsJ (Amr) | This study |
| pBsPTA1 | pTEV5 expressing B. subtilis 6×His-tagged PTA (Amr) | Escalante-Semerenaa |
a
Provided by Jorge C. Escalante-Semerena at the University of Georgia (Athens, GA).