FIG 3.

Expression changes in response to ERG11 alleles. A. Levels of mRNA for PDR1, CDR1, and ERG11 were assayed by RT-qPCR in the indicated ERG11 mutant strains during growth in the absence (Control) or presence of fluconazole stress (Fluconazole). Fluconazole treatment was 20 μg/ml for 2 h. B. Whole cell protein extracts were prepared from the same strains in A. These extracts were analyzed by Western blotting using the indicated antisera against Cdr1, Pdr1, Erg11, Upc2A or Tubulin. Equal loading and transfer of these extracts was ensured by staining the nitrocellulose membrane with Ponceau S prior to antisera incubation. C. Quantitation of 4 replicates of this Western blotting is shown with the data presented as the ratio of each strain relative to the wild-type cells. D. RT-qPCR analysis of ERG gene expression in the DM ERG11 mutant background relative to the wild-type level. HMGCoA (HMG-CoA reductase) refers to the HMG1 or CAGL0L11506g locus.