FIG 5.

Growth phenotypic comparison of DM ERG11 with gain-of-function (GOF) alleles of PDR1. A. Isogenic mid-log phase C. glabrata strains containing ERG11 wild-type or DM alleles, PDR1 null (pdr1Δ), wild-type PDR1 (PDR1::loxP), or two GOF alleles of PDR1 D1082G and R376W were serially diluted and spotted on YPD agar plates supplemented with or without fluconazole (20 μg/ml). Plates were incubated at 30°C and photographed. B. Fitness comparisons between the DM ERG11 strain compared with wild-type (B), the GOF D1082G (C) and R376W (D) PDR1 mutant strains. Equal number of mid-log phase cells from each strain were mixed together (Pretreatment). Cultures were then allowed to grow in the absence (YPD) or presence of azole drug (Fluconazole) (20 μg/ml). At the end of incubation, samples of each culture were plated and final distribution of each strain determined by plating. Deviations from the starting 50:50 mix of strains would indicate a relative fitness advantage for one strain versus the other.