FIG 6.

Relative gene expression responses to the DM ERG11 or two different GOF PDR1 strains. A. Strains varying at the ERG11 or PDR1 loci as indicated were grown in the presence or absence of fluconazole as in Fig. 3 and processed for RT-qPCR analyses of the indicated mRNAs. Fluconazole was included at 20 μg/ml. B. The strains above were analyzed by Western blotting as in Fig. 3 using antisera for Cdr1, Pdr1, Erg11, Upc2A or Tubulin (Tub). The membrane was stained after transfer with Ponceau S as above. Quantitation of 4 western experiments is provided on the right hand side of the figure.