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The AldF subunit is responsible for membrane binding of the AldFGH complex. G. diazotrophicus strain MR17, referred to as the reference strain, and strain MR25 (MR17 ΔaldF), referred to as the ΔaldF strain, were cultivated in 100 mL YPG medium. The membranes (red) and the crude soluble fraction (blue) were separated by ultracentrifugation. Acetaldehyde:ferricyanide oxidoreductase activity was determined at pH 6.0. Mean values and standard deviations from triplicate assays are shown.
