TABLE 2.
Summary of purification of the AldGH subcomplex from recombinant Acetobacter pasteurianus mNS4a
| Purification step | Total protein concn (mg) | Sp act (U/mg) | Total activity (U) | Yield (%) |
|---|---|---|---|---|
| Crude soluble fraction | 1,000 | 0.64 | 660 | 100 |
| DEAE-Toyopearl | 95 | 6.1 | 580 | 88 |
| Phenyl-Toyopearl | —b | —b | 280 | 42 |
| Hydroxyapatite | 1.8 | 100 | 180 | 28 |
a
Acetaldehyde:ferricyanide oxidoreductase activity was measured at pH 5.0.
b
—, accurate protein content was not determined because of the presence of ammonium sulfate that may affect the Lowry method.