TABLE 3.
Summary of the properties of ALDH complexes purified from Gluconacetobacter diazotrophicus strain PAL5
| Property | Value in study |
|
|---|---|---|
| Gomez-Manzo et al.b | This study | |
| Specific FC3− reductase activity (U mg−1) | 800 | 300 |
| Optimum pH for FC3− reductase activity | 3.5 | 4.0 |
| Presence of ubiquinone reductase | No | Yes |
| Presence of small subunit | No | Yes |
| Size of subunit detected by heme staining | Middle | Middle |
| No. of hemes c | 3 | 3a |
| No. of hemes b | 1 | None |
| Reduction of cytochromes by the substrate | No | Yes |
a
Determination of heme c showed 2.1 mol per mol of the AldFGH complex. However, we suggest that there is 3 mol of heme c per mol of the AldFGH complex, even though the nearest integer was 2, because impurities in the enzyme preparation may decrease the experimentally observed value.
b
See reference 8.