TABLE 5.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Description | Source and/or reference |
|---|---|---|
| Bacterial strains | ||
| Gluconacetobacter diazotrophicus | ||
| ATCC 49037 | Wild type; synonym of PAL5 | ATCC; 37 |
| MR17 | PAL5 ΔaldSLC ΔadhAB ΔpqqABCDE | 10 |
| MR25 | PAL5 ΔaldSLC ΔadhAB ΔpqqABCDE ΔaldF | This study |
| Acetobacter pasteurianus | ||
| SKU1108 | Wild type; synonym of NBRC101655 | 38 |
| mNS4 | SKU1108 ΔadhAB ΔaldSLC ΔaldFGH | Nina et al., unpublished |
| Plasmids | ||
| pT7Blue | Cloning vector; Apr | Novagen |
| pRK2013 | Plasmid-mediated plasmid transfer; Kmr | 30 |
| pK18mobsacB | Suicide vector; mob sacB; Kmr | 28 |
| pCM62 | Broad-host-range plasmid; mob lacZα; Tcr | 29 |
| pMRG | pK18mobsacB with a 2-kb fragment containing the aldF allele | This study |
| pTM8 | pCM62 aldGH | This study |
| pTM10 | pCM62 aldH | This study |
| pTM14 | pCM62 aldF with the putative aldFGH promoter in the orientation opposite that of the lac promoter | This study |
| pTM15 | pT7Blue aldFGH with a putative aldFGH promoter | This study |
| pTM16 | pCM62 aldFGH with a putative aldFGH promoter in the orientation opposite that of the lac promoter | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.