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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: Mol Cancer Res. 2020 Oct 26;19(2):223–239. doi: 10.1158/1541-7786.MCR-20-0464

Figure 4: Aggressive tumor progression and shorter overall survival of ALDH+ tumor bearing mice.

Figure 4:

Aldefluor stained and FACS sorted HJSD-DIPG 007 luciferase expressing cells (DIPG 007-luc) were intracranially implanted into NSG mice. A. Data represents average total flux per implant group ± SEM. P-values calculated with unpaired t-tests. B. Representative BLI images of each implant group at 3 months. C. Ex vivo BLI of extracted brains acquired at the time of sacrifice. D. Kaplan-Meier survival curves for ALDH+ (n=13), ALDH- (n=9) and unsorted (n=6) mice, respectively with p-values from log-rank (Mantel-Cox) tests. E. Representative cryo-fluorescence tomography (CFT) of a brain extracted from a mCherry expressing and ALDH+ sorted DIPG 007 mouse at day 90 post-implantation. Image presented as maximum intensity projections (MIPs) at the 555excitation acquisition. F. Representative H&E and immunohistochemical staining of extracted brain sections. Images acquired at 40x, scale bar= 100 μm. G.-I. IHC quantification using ImageJ software. One-way ANOVA with p values for multiple comparisons from Tukey’s post-hoc test.