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. Author manuscript; available in PMC: 2023 Mar 14.
Published in final edited form as: Angew Chem Int Ed Engl. 2022 Jan 27;61(12):e202115704. doi: 10.1002/anie.202115704

Figure 2.

Figure 2.

In vitro chemiluminescence ratiometric O2 imaging of the IrCL probes under aerated or degassed conditions. (A)−(C) Chemiluminescence images obtained for IrCL-1, IrCL-2 and IrCL-3, respectively, at different wavelengths, depicting the intensity increase of the triplet emission under degassed conditions. (D) Comparison of the flux ratios (total fluxtriplet/total fluxsinglet) recorded at the relevant wavelengths for IrCL-1, IrCL-2 and IrCL-3, respectively. The flux ratio or ratiometric response is significantly quenched under aerated conditions, especially for IrCL-1, highlighting the O2 sensitivity. [IrCL] = 20 μM in 50% DMSO-PBS buffer (10 mM, pH = 7.4). Error bars are ±SD for n = 3 independent experiments. Exposure time = 5 s.