Platelet aggregation was induced by indicated amounts of PAF (A, E & F), ADP (B, G & H), thrombin (C, I & J) or collagen (D, K & L) with or without sAGP-1 and nAGP-1. (A) inset shows the effect of PAF (positive control), sAGP-1 and nAGP-1 on platelet aggregation. Each set of experiments were carried out with platelets from the same donor as well as repeated with platelets from at least three other donors. All assays were performed using Chrono-log aggregometer and the traces were recorded using AGGRO/LINK 8 ver.1.0.1 software. Representative trace of three independent experiments is shown here. The aggregation traces were merged using Wacom graphics pad. The data shown are mean ± SEM (n = 3). ***p<0.0001, *p<0.01 as determined by one – way ANOVA.