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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: J Leukoc Biol. 2020 Oct 18;109(5):915–930. doi: 10.1002/JLB.3A0720-422R

Fig. 7: Inhibition of PAF and ADP-induced platelet aggregation by sAGP-1 is mediated by a cAMP-dependent pathway:

Fig. 7:

(A) Quantification of cAMP levels in plaelets. Washed platelets were stimulated with PAF, ADP or Thrombin with or without sAGP-1. After incubation, the cells were collected by centrifugation and then assayed for cAMP by ELISA. The data represents results from two different experiments. (B) Phosphokinase western blot. The phosphorylation status of the stated kinases treated, or not, with the indicated agonist as in the preceding panel was visualized by western blotting. Phosphorylation of Akt, p38, ERK, or VASP was normalized using total β-actin. (C – F) Densitometric analyses of phosphokinase immunoblots. Densitometric analysis of blots obtained from 3 experiments was done using ImageJ software (ver. 1.51j8). The data shown are mean ±SEM ***p < 0.001, **p < 0.01 and *p < 0.05 as analysed by one – way ANOVA.