Figure 4.

24a promotes the expression of DRs. Cell lysates were prepared from Detroit 562 cells treated with 24a for 24 h at the indicated doses. (A) Human Apoptosis Array permitted the probing of 35 apoptosis-related proteins by antibodies spotted in duplicates on nitrocellulose membranes. Representative images of chemiluminescence spots corresponding to DR4 and DR5 are demonstrated. (B) Western blot results depicting DR expression levels. Representative blots are shown, with GAPDH depicting the typical loading control. (C) Relative protein expressions are quantified from blots determined in B and Fig. 3E for c-PARP. (D and E) Summary of the relative protein expressions of total p53, p53 proteins at various post-translational modification sites, p21, p63 and p73 determined from representative blots shown in Fig. S5. Values represent the mean ± SD of n=3, with the treatment values compared with that of the corresponding mock values. ^*P<0.05 vs. 0 µM control. DR, death receptor; c-, cleaved; PARP, poly (ADP-ribose) polymerase 1; TNFR, tumor necrosis factor receptor.