| Cell line (H. sapiens) |
T HESCs |
ATCC |
CRL-4003RRID: CVCL_C464
|
|
| Antibody |
Anti-SOX4(Rabbit polyclonal) |
Abcam |
Cat # ab80261 RRID:AB_1658989
|
WB (1:600)ICC (1:1000)IF (1:300) |
| Antibody |
Anti-SOX4(Rabbit polyclonal) |
Diagenode |
Cat # C15310129 |
IP (1:100) |
| Antibody |
Anti-PGR(Rabbit monoclonal) |
CST |
Cat # 8757 RRID:AB_2797144
|
WB (1:500)ICC (1:3000)IF (1:200)IP (1:50)ChIP (1:50) |
| Antibody |
Anti-HERC4(Rabbit polyclonal) |
Protein tech |
Cat # 13691-1-AP RRID:AB_10596480
|
WB (1:1000)ICC (1:1000)IF (1:300) |
| Antibody |
Anti-FOXO1(Rabbit polyclonal) |
Abcam |
Cat # ab39670 RRID:AB_732421
|
WB (1:1000)ICC (1:3000) |
| Antibody |
Anti-IGFBP1(Rabbit polyclonal) |
Abcam |
Cat # ab228741 |
WB (1:1000)IHC (1:500) |
| Antibody |
Anti-Ub(Mouse monoclonal) |
CST |
Cat # 3936 RRID:AB_331292
|
WB (1:1000) |
| Antibody |
Anti-HA(Rabbit monoclonal) |
CST |
Cat # C29F4 |
WB (1:1000)IP (1:100)ChIP (1:100) |
| Antibody |
Anti-Myc(Mouse monoclonal) |
CST |
Cat # 2276 RRID:AB_331783
|
WB (1:1000) |
| Antibody |
Anti-Flag(Mouse monoclonal) |
Sigma |
Cat # F9291 RRID:AB_439698
|
WB (1:1000) |
| Antibody |
Anti-GAPDH(Rabbit polyclonal) |
Abmart |
Cat # P30008
|
WB (1:2000) |
| Chemical compound, drug |
E2 |
Sigma |
E8875 |
Final concentration: 10 nM |
| Chemical compound, drug |
MPA |
Sigma |
M1629 |
Final concentration: 1 μM |
| Chemical compound, drug |
cAMP |
MCE |
HY-B0764 |
Final concentration: 0.5 mM |
| Chemical compound, drug |
MG132 |
Selleck |
S2619 |
Final concentration: 20 μM |
| Chemical compound, drug |
3-MA |
Selleck |
S2767 |
Final concentration: 20 mM |
| Chemical compound, drug |
CHX |
MedChemExpress |
HY-12320 |
Final concentration: 20 μg/ml |
| Chemical compound, drug |
Insulin–transferrin–selenium |
Thermo Fisher |
51500-056 |
Final concentration: 1% |
| Chemical compound, drug |
Puromycin |
MedChemExpress |
HY-15695 |
Final concentration: 500 ng/ml |
| Chemical compound, drug |
RNAi MAX |
Thermo Scientific |
13778030 |
7.5 μl/6-well |
| Chemical compound, drug |
Lipofectamine 2000 |
Thermo Scientific |
11668030 |
5 μl/6-well/2500 ng DNA |
| Commercial assay or kit |
Dual-Luciferase Reporter Assay System |
Promega |
E1910 |
|
| Commercial assay or kit |
ChIP-IT high Sensitivity |
Active Motif |
53,040 |
|
| Commercial assay or kit |
KAPA DNA HyperPrep Kit |
Roche |
KK8502 |
|
