| Gene (Drosophila melanogaster) |
Early gene 23 (E23) |
Flybase |
FBgn0020445 |
|
| Gene (Drosophila melanogaster) |
Ecdysone-induced protein 74EF(E74) |
Flybase |
FBgn0000567 |
|
| Gene (Drosophila melanogaster) |
Ecdysone-induced protein 75B(E75) |
Flybase |
FBgn0000568 |
|
| Cell line (D. melanogaster) |
S2-DRSC |
Drosophila Genomics Resource Center (DGRC) |
RRID: CVCL_Z992
|
|
| Chemical compound, drug |
20-hydroxyecdysone (20E) |
Sigma-Aldrich |
Cat#: H5142 |
|
| Chemical compound |
Flavopiridol hydrochloride |
Tocris Bioscience |
Cat#: 3,094 |
|
| Chemical compound |
Triptolide |
Sigma-Aldrich |
Cat#: T3652 |
|
| Chemical compound |
Fugene HD |
Promega |
Cat#: E2311 |
|
| Chemical compound |
Trizol |
Ambion |
Cat#: 15596018 |
|
| Commercial kit |
Megascript T7 kit |
Ambion |
Cat#: AM1334 |
|
| Commercial kit |
Purelink RNA mini kit columns (; 12183018 A) |
Ambion |
Cat#: 12183018 A |
|
| Commercial kit |
QuantiTect RT-PCR |
Qiagen |
Cat#: 205,311 |
|
| Commercial kit |
PowerSYBR Green PCR Master Mix |
Applied Biosystems |
Cat#: 4367659 |
|
| Chemical compound |
Atto-565 |
Sigma-Aldrich |
Cat#: 72,464 |
|
| Chemical compound |
ProLong Gold mountant media |
ThermoFisher |
Cat#: P36930
|
|
| MATLAB |
|
MathWorks |
|
https://www.mathworks.com/
|
