Figure 2.
AOAA alleviated DSS-induced colitis in mice and restored mRNA expression of epithelial barrier. (a) Colonic sections were stained with H&E (original magnification ×200). (b) Histopathological scores of colonic sections. The mRNA expression of (c) ZO-1, (d) occludin, (e) claudin-1, (f) E-cadherin, and (g) vimentin. (h) The expressions of these proteins were tested by immunohistochemistry in experimental colitis in mice. One representative experiment of three is shown. Data are represented as mean ± SD (n = 5) (#P < 0.05 vs. the H2O+PBS group; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 vs. the DSS+PBS group).