Figure 2.

CircRHOT1 represses autophagy and in the OA rat model. (a)–(e) The OA rat model was constructed by receiving ACLT, and the OA rats were treated with circRHOT1 shRNA. (a) The expression of circRHOT1 was detected by qPCR. (b, c) The apoptosis was measured by TUNEL staining. Magnification, ×40. (d, e) The articular cartilage degeneration was presented by Mankin score and OARSI score. (f, g) The serum levels of CTX-II and COMP were measured by ELISA assay. (h) The expression of collagen II and aggrecan was detected by Western blot analysis. (i) The protein expression of LC3 and beclin1 was detected by Western blot analysis. Mean ± SD, ^∗∗P < 0.01.