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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Exp Eye Res. 2022 Jan 2;215:108918. doi: 10.1016/j.exer.2021.108918

Fig. 4. Increased respiration and ATP generation in senescence and inhibition by mini Cry.

Fig. 4.

Sub-confluent RPE cells were treated with 500 μM H2O2 in SFM for 2 h, which was replaced with fresh medium containing mini Cry or Scr peptide. H2O2 treatment was repeated the next day. Cells were maintained in 10% FBS for 4 d. Mitochondrial bioenergetics were analyzed by Seahorse XFe96. H2O2-induced RPE senescence significantly increased basal respiration, ATP production and maximal respiration and mini Cry co-treatment dose-dependently reduced mitochondrial bioenergetic parameters. Values are means ± SE (N= 8-10). NS- Not significant; * p< 0.05. ** p< 0.01, *** p< 0.001.