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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Exp Eye Res. 2021 Dec 23;215:108902. doi: 10.1016/j.exer.2021.108902

Figure 2.

Figure 2.

qRT-PCR, western blot, densitometry analysis of CBS and CSE gene and protein expression in normal canine control and, xlpra2, rcd1 and crd2 diseases taken in 7.7–13.6 weeks of age time window (see Table 1). CBS (a) and CSE (b) mRNA expression was normalized with GAPDH gene and expressed as fold change. The results showed an increase in CBS but not CSE expression in the 3 retinal disease models; CBS increase was significant in rcd1 and crd2. CBS protein levels in the rcd1 and crd2 groups were significantly higher than normal samples (c and e). CSE protein levels did not change in affected dogs compared to normal dogs (d and f). The results are shown as the means ± SD. The significance of difference among groups was evaluated by a one-way ANOVA with a post hoc ‘Tukey’s test. (One-way ANOVA, *P < 0.05).