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Immunohistochemical analysis of acrolein expression (red fluorescence) in normal and mutant retinas. At the time of active degeneration, there was a retina-wide increase in acrolein expression (E, H, K). After photoreceptor degeneration is almost completed, intensity decreases but remains elevated in rcd1 and crd2 retinas (I, L). DAPI (blue) nuclear stain. Scale bar: 20 μm; PR: Photoreceptor cells, ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer.
