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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Exp Eye Res. 2021 Dec 23;215:108902. doi: 10.1016/j.exer.2021.108902

Figure 9.

Figure 9.

qRT-PCR (a), western blot (b), and densitometry analysis (c) of BCL2 expression in normal and mutant canine retinal samples taken in 7.7–13.6 weeks of age time window (see Table 1). (a) mRNA expression, normalized to GAPDH, showed increased expression in xlpra2 and rcd1, and the increase was significant in rcd1. (b, c) western analysis with an anti BCL2 antibody. (c) Normalized BCL2 protein expression as fold increase over normal samples normalized with a GAPDH internal control. BCL2 protein levels are decreased in the 3 different mutant retina groups, significant in xlpra2 and crd2. The significance of difference among groups was evaluated by a one-way ANOVA with a post hoc Tukey’s test. (One-way ANOVA, *P < 0.05).