Table 2.
PCR primer sequences, optimal amplification cycles, optimal annealing temperature, and product size
| Gene | Accession number | Primer sequence | Optimal condition |
|---|---|---|---|
| CSE | XM_537115 | F: 5-GCAATGGAATTCTCGTGCCG-3’ R: 5-ATGCAAAGGCCAAACTGTGC-3’ |
40 cycles Annealing 60°C |
| CBS | XM_014109845 | F: 5-GGCTGGAAAGGTGCGGCCAT-3’ R: 5-CTTGCTGGACATGCCATTGCTG |
40 cycles Annealing 60°C |
| NOS2 | NM_001313848.1 | F: 5-ACTTCTCCTGGCTGTCTCT-3’ R: 5-GGCCTACTGACTTCACTTATG-3’ |
40 cycles Annealing 60° |
| BCL2 | NM_001002949.1 | F: 5-ACTTCTCCTGGCTGTCTCT-3’ R: 5-GGCCTACTGACTTCACTTATGG-3’ |
40 cycles Annealing 60° |
| GAPDH | NM-001003142 | F: 5-CTGTCGAGTCGCGTCCACCC-3 R: 5-ACATGCCGGAGCCGTTGTCG-3’ |
40 cycles Annealing 60°C |