Table 3.
Antibodies used for western blot and immunohistochemistry.
| Antigen | Antibody | Host | Type | Immunogen species | Dilution for WB | Dilution for IHC |
|---|---|---|---|---|---|---|
| CBS | Proteintech, 14787–1-AP | Rabbit | IgG Polyclonal | Human | 1:1000 | 1:500 |
| CSE | Proteintech, 60234–1 -Ig | Mouse | IgG1 Monoclonal | Human | 1:1000 | 1:500 |
| NOS1 | Proteintech, 18984–1-AP | Rabbit | IgG Polyclonal | Human | 1:1000 | 1:200 |
| NOS2 | Novus NB300–605SS | Rabbit | IgG Polyclonal | Mouse | - | 1:200 |
| 4HNE | ALPHA DIAGNOSTIC INTERNATIONAL HNE11-S | Rabbit | IgG Polyclonal | Free HNE coupled with Keyhole limpet hemocyanin | 1:1000 | 1:200 |
| Acrolein | Novus NBP2–59359 | Mouse | IgG1 Monoclonal | Synthetic | - | 1:200 |
| GAPDH | Proteintech, 10494–1-AP | Rabbit | IgG Polyclonal | Human | 1:10000 | - |
| GAPDH | Proteintech, 60004–1-lg | Mouse | IgG2b Monoclonal | Human | 1:10000 |