Extended Data Fig. 6. Effects of mTOG-Ψ and siPAIP1 on HC and HR-MDS HSPC in vitro expansion.
a, Graph shows mTOG quantification in HC and HR-MDS HSPC cells after transfection with mTOG-Ψ or SCR-Ψ oligo (3 days). n=3 independent biological experiments (except for SCR-Ψ of HR-MDS, where n=2) subject to limited patient sample availability. b, Graph shows quantification of CD34+ cells in HC and MDS-HSPC transduced with mTOG-Ψ or SCR. ns, no statistically significant difference was observed (Welch’s two-tailed t test). n=3 independent biological experiments. c, Graph shows no changes in HC and MDS-HSPC cell viability in the presence of mTOG-Ψ or SCR-Ψ oligo. d, Steady-state HC and MDS-HSPC proliferation is not affected by mTOG-Ψ in culture. Graphs show number of HC and MDS-HSPC at different days in the presence or absence of mTOG-Ψ. e, No significant changes in proliferation of HR-MDS HSPC were detected at different days upon SCR-Ψ, siPAIP1 and the combination mTOG-Ψ/siPAIP1 treatment For each patient, n=3 independent experimental replicates. Data is shown as mean ± SD.