Extended Data Fig. 8. Effects of PUS7 depletion and mTOG-Ψ treatment on HC HSPC colony-forming capacity.
a, Top, representative images of CFUs obtained using HSPC isolated from HC and HR-MDS patients treated with mTOG-Ψ or SCR-Ψ oligos. Bottom, graph shows FC colony number obtained HC HSPC transduced with SCR-Ψ, mTOG-Ψ, siPAIP1 and the combination mTOG-Ψ/siPAIP1 at day 15. ns, no statistical significance (one-way ANOVA with multiple comparison). Data is shown as mean ± SD. n=2-8 patient samples as indicated, dots show individual patients. b, Protein analysis show reduced levels of EIF6, RPL23 and RPL29 in CFUs obtained from a HSPC of an HR-MDS patient (MDS304) in the presence of mTOG-Ψ and siPAIP1. In contrast, no differences were detected in CFUs obtained from HC HSPC (NBM141). Data represent two independent healthy and MDS patients in duplicate experiments.