Extended Data Fig. 9. mTOG-Ψ promote HR-MDS HSPC multi-lineage differentiation.
a, Graph shows mTOG quantification in HC and HR-MDS HSPC cells after transfection with mTOG-Ψ or SCR-Ψ oligo following erythroid (Ery) and myeloid (Mye) differentiation (day 15). Data show two independent biological experiments. b, mTOG-Ψ promote expansion of HR-MDS cells in erythroid (top) and myeloid (bottom) differentiation media. No effects were observed in n=3 individual HC patient samples, (two-sided t test). Graphs show number erythroid (CD235a+/CD36+) and myeloid (CD66b+/CD33-) cells. during differentiation in the presence or absence of mTOG-Ψ. c, PAIP1 downregulation increase erythroid (top) and myeloid (bottom) HR-MDS cells expansion. Differentiation is not further enhanced by co-transfection with mTOG-Ψ oligos. For each patient, n=3 independent experimental replicates. Data is represented as mean ± SD.