Figure 5.

Innate memory response induced by H. pylori in monocytes. (A) Experimental setup of the monocyte innate memory model. Human monocytes were infected with H. pylori (Hp) at the indicated MOI for 24 h, then washed and incubated with fresh medium containing antibiotics for 6 days. Medium was then replaced and cells were challenged with LPS (5 ng/mL) for 24 h. (B) Cytokine and chemokine secretion by human monocytes analyzed 24 h post-infection with H. pylori at MOIs 0.2, 1 and 5 by Multiplex assay. (C) After six days of resting, monocytes were either left untreated (empty bars) or challenged with LPS (10 ng/mL; filled bars). Cytokine and chemokine secretion was measured 24 h post-challenge via Multiplex assay. Bars represent mean + SD of at least four individual donors. For statistical analysis, RM-ANOVA with Dunnet’s post-hoc test (B) and Šidak’s post-hoc (C) test were performed (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001).