FIGURE 1.

MICA/B release is increased from senescent cells. (A) SH-SY5Y cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control, and after 72 h, cellular senescence was evaluated by SA-β-gal staining. Senescent cells are shown by blue staining (magnification: ×20). (B) Cell cycle progression was assessed by flow cytometry. (C) Four different neuroblastoma cell lines were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control, and after 72 h, (D) MICA and MICB concentrations in the supernatant were evaluated by ELISA. Data represent the mean ± SEM of three independent experiments. *p < 0.05.