FIGURE 6.

MALAT1 silencing inhibits MICA/B release and inhibits the immune escape of senescent cells. (A) Following co-transfection of an miR-92a-3p inhibitor and si-MALAT1 into IMR-32 cells, cells were treated with MLN8237 to induce cell senescence, and ELISA was performed to detect MICA/B levels in the culture supernatant. (B) Flow cytometry analysis of MICA/B expression on the cell surface. (C) Killing efficiency was detected by the calcein-AM method in co-cultures at effector:target cell ratios of 5:1, 10:1, and 20:1. Cells co-transfected with different vectors were used as target cells, normal human NK cells were used as effector cells. Data represent the mean ± standard error of the mean of three independent experiments. *p < 0.05.