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. 2022 Mar 15;12:105. doi: 10.1038/s41398-022-01821-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Primer sequences of genes used for qPCR experiments. All primers were validated in-house, and expression levels of GAPDH (reference gene) were not significantly different between bHRs and bLRs (Supplemental Materials, Fig. S1). B Elevated hippocampal expression (Log(2) fold change) for several genes within the classical complement cascade was found in bLRs compared to bHRs. Data are expressed as mean ± SEM, with scatter plots overlaid to show individual data points. Welch’s t-tests: bHR v. bLR, ***C1qA: t_17.92 = 33.713, p < 0.001; ***C1qC: t_16.63 = 20.305, p < 0.001; *C3: t_14.52 = 5.258, p = 0.037; **Cd11b: t_17.89 = 12.874, p = 0.0012.