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. 2022 Mar 15;12:105. doi: 10.1038/s41398-022-01821-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — High-magnification photomicrographs (63x, oil) of typical ramified microglia from bHR (A) and bLR (B), with Neurolucida reconstructions for each cell shown in (C) and (D), respectively. Scale bar = 20 µm; Concentric circles applied at 5 µm increments in C and D for length comparisons. Reconstructions were analyzed using Neurolucida Explorer Software (MicroBrightField Bioscience). The main measurements are summarized here, with a full listing provided in Supplemental Materials. Although microglia from each line had similar totals of primary branches (E), bLR microglia had longer branches in CA1 (**t₇₄ = 3.322, p = 0.001) and CA3 (***t₇₄ = 5.765, p < 0.001) (F), and more branch points across all regions (***CA1: t₇₄ = 6.267, p < 0.001; ***CA3: t₇₄ = 7.012, p < 0.001; ***DG: t₇₄ = 4.022, p < 0.001) (G), compared to microglia from bHRs. H Convex hull calculations, which reflect the overall territory area of the cell, were larger in bLRs in CA1 (***t₇₄ = 3.909, p < 0.001) and CA3 (***t₇₄ = 4.869, p < 0.001). Data are expressed as mean ± SEM, with scatter plots overlaid to show individual data points.