Skip to main content
. 2022 Mar 15;8:24. doi: 10.1038/s41531-022-00289-9

Fig. 2. Genotype of the adaptive immune system has primacy over the genotype of the brain in regard to induction of LPS-induced neuronal loss.

Fig. 2

a Double mutant chimeras (WT(GFP)->Rag1/ R1441G) expressing wt LRRK2 in T- and B-cells show completely rescued TH cell loss compared to LPS-treated R1441G mice. Data are mean ± SEM, n ≥ 4. *p < 0.05 vs WT or vs group indicated on the graph. b Rostro-caudal gradient of DA cell loss seen in R1441G mice was restored in double mutant chimeras carrying wt immune cells and mimicked in reversed chimeras carrying mutant T- and B-cells. c Reversed chimeras expressing mutant LRRK2 only in adaptive immune cells develop SNpc TH cell loss 7 days after LPS treatment similar to LPS-treated R1441G mice. Note that LPS-treated control Rag1−/− mice transplanted with GFP+/+ BM did not develop LPS-induced DA cell loss. Data are mean ± SEM, n ≥ 4. *p < 0.05 vs WT or vs group indicated on the graph. d Representative images of TH-positive DA neurons in the SNpc of WT, R1441G mice, and chimeras 7 days post-LPS injection. Sections are matched at the same level of the SN (Bregma −3.08–3.16 mm)112. Scale bar = 100 μm.