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. 2022 Mar 15;13:1346. doi: 10.1038/s41467-022-28962-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Example STORM render image of MVI and RNAPII-pSer5 under normal conditions and serum-treated conditions (scale bar 2 μm). B Co-localisation analysis of MVI and RNAPII-pSer5 clusters under normal and serum-treated conditions. Inset is a representative cluster colocalization heatmap whereby DoC values of 1 are perfectly colocalised and −1 are separated from each other. Individual data points represent the percentage of each protein which is colocalized based on DoC threshold of above 0.4 (n = 13) (see Fig. S6 for example histograms). The values represent the mean from the ROIs for each protein (Only statistically significant changes are highlighted ****p < 0.0001 by two-tailed t test compared to normal conditions for each protein). C Cluster analysis of RNAPII-pSer5 nuclear organisation under normal and serum-treated conditions. The data are broken down into myosin VI colocalized and non-colocalized clusters. Individual data points correspond to the average value for a cell ROI (n = 14). The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by two-tailed t test compared to normal conditions). D Cluster analysis of myosin VI nuclear organisation under normal and serum-treated conditions. The data are broken down in to RNAPII-pSer5 colocalized and non-colocalized clusters. Individual data points correspond to the average value for a cell ROI (n = 14). The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted **p < 0.01; ***p < 0.001; ****p < 0.0001 by two-tailed t test compared to normal conditions).