Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 15;13:1346. doi: 10.1038/s41467-022-28962-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A Cartoon depicting simultaneous acquisition of 9 focal planes covering 4 μm to perform live-cell 3D single-molecule tracking of RNAPII. B Example render of 3D single-molecule trajectories under normal and TIP-treated conditions. Further examples are shown in Supplementary Fig. 15. C Example trajectory of a diffusive and spatially confined molecule which can be identified in (B). D Plot of Halo-RNAPII diffusion constants under the stated conditions derived from fitting trajectories to an anomalous diffusion model, as described in methods. Individual data points correspond to the average value for a cell ROI (n = 100). The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted ****p < 0.0001 by two-tailed t test compared to normal conditions). E Example histogram of diffusion constants arising from a single cell. The dotted line represents the threshold applied to segregate static and dynamic molecules. F Using the threshold defined in (E), trajectories were plotted as a ratio of mobile and immobile species. Individual data points correspond to the average value for a cell ROI. The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted ****p < 0.0001 by two-tailed t test compared to normal conditions). NLS-R62D Actin, NLS CBD and NLS Motor refer to tracking of RNAPII following transfection of these constructs. NLS CBD and NLS motor were transfected in to Halo-RNAPII cells. G Plot of Halo-MVI diffusion constants under the stated conditions derived from fitting trajectories to an anomalous diffusion model, as described in methods. Individual data points correspond to the average value for a cell ROI (n = 100). The values represent the mean from the ROIs for each condition (Only statistically significant changes are highlighted ****p < 0.0001 by two-tailed t test compared to normal conditions).