Figure 2.

Activity of single components, combinations between compounds and EOs from Thymbra capitata on a 48 h Gardnerella sp. UM241 biofilm. (a) Effect on biofilm biomass reduction. Results are presented in mean percentage (%) of biofilm biomass reduction. (b) Effect on biofilm cells culturability. Results are represented as mean of Log (CFU.mL⁻¹) with a limit of detection of Log=3. Primary colors were selected to represent the individual components and the mixtures represent the color combination of the individual components. Statistical analysis was performed using one-way ANOVA (n ≥ 3). In panel (a), differences are showed, when p < 0.05, by asterisk symbol when comparing with 5 µL/mL α-terp; hash symbol when comparing with 5 µL/mL γ-terp; bullet symbol when comparing with 0.02 µL/mL carv + 2.5 µL/mL ρ-cym; filled square symbol when comparing with 0.02 µL/mL carv + 1.25 µL/mL linl and filled diamond symbol when comparing with 0.02 µL/mL carv + 2.5 µL/mL ρ-cym + 0.32 µL/mL linl. All conditions are statistically different from the 48 h-biofilm control except for the cases marked (a). In panel (b), differences are represented by asterisk symbol when comparing with control 48 h and hash symbol when comparing with control 72 h, when p < 0.05.