Figure 3.

Activity of EO Car. and two combinations among compounds on a 48 h biofilm of G. vaginalis UM137, G. piotii UM035, G. leopoldii UGent 09.48 and G. swidsinskii GS 9838–1. a: Effect on biofilm biomass reduction. Results are presented in percentage (%) of biofilm biomass reduction. Error bars represent s.e.m. Statistical differences were found for all the conditions when compared with the 72 h-biofilm control, except for G. leopoldii where no differences were found (a) (t-test, n = 4). Statistical differences were found in G. leopoldii, between conditions A and C (asterisk symbol) (t-test, n = 4). (b) Effect on biofilm cells culturability. Results are represented as mean of Log (CFU.mL⁻¹) with a limit of detection of Log = 3. Error bars represent s.d. Statistical analysis was performed using t-test (n = 4). Differences are represented when p < 0.05, when compared with control 72 h of G. vaginalis UM137 (asterisk symbol), G. piotii UM035 (hash symbol), G. leopoldii UGent 09.48 (bullet symbol) and G. swidsinskii GS 9838-1 (filled square symbol).