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. 2022 Mar 2;13:844610. doi: 10.3389/fimmu.2022.844610

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Copyright © 2022 Dhande, Bagul and Thakar

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of killing of reactivated HIV-1 latent reservoirs by the generated ADCC antibodies. (A) The line diagram shows the % p24-expressing CD4+CD45RO+ resting memory T cells (Y-axis) in unstimulated cells, after Env C stimulation and after the addition of antibodies generated from R-ADCC LTNPs (black dots) and NR-ADCC LTNPs (red dots) to the Env-stimulated cells (X-axis). (B) The scatter plot shows % p24 reduction (Y-axis) in five R-ADCC LTNPs (R1, R3, R4, R6, and R7) and 2 NR-ADCC LTNPs (NR1 and NR2) (X-axis). (C) The box and whiskers plots % NK cell activation (Y-axis) in unstimulated cells, after Env C stimulation and after the addition of antibodies generated from R-ADCC LTNPs and NR-ADCC LTNPs to the Env-stimulated cells (X-axis). The Wilcoxon matched-pairs t-test was used to analyze the data and p ≥0.05 was considered to be significant. The ** represents P ≤ 0.01.