Table 1.
Characteristics of in-vitro-generated HIV-specific antibodies.
| Sample ID | ADCC activity (% PKH26 uptake) | Latency reduction | |||
|---|---|---|---|---|---|
| Env C | Env B | % p24 reduction | % NK cell activation | ||
| R1 | 33.4 | 34.4 | 64.99 | 23.67 | |
| R2 | 35 | 36.5 | Not assessed | ||
| R3 | 43 | 31.9 | 62.50 | 50.27 | |
| R4 | 32.5 | 26.9 | 64.86 | 26.23 | |
| R5 | 27.9 | 53.8 | Not assessed | ||
| R6 | 53.8 | 47.7 | 61.05 | 21.56 | |
| R7 | 48.2 | 33.4 | 56.37 | 31.05 | |
| R8 | 37.6 | 39.5 | Not assessed | ||
| R9 | 62.1 | 67.8 | |||
| Median | 37.6 | 36.5 | 62.5 | 26.23 | |
| IQR | 32.95–51 | 32.65–50.75 | 58.71–64.92 | 22.62–40.66 | |
| Range | 27.9–62.1 | 26.9–67.8 | 56.37–64.99 | 21.56–50.27 | |
| NR1 | 8 | 5.9 | 9.86 | 7.14 | |
| NR2 | 9.7 | 2.5 | 17.81 | 13.15 | |
| Median | 8.85 | 4.2 | 13.84 | 10.15 | |
The table shows the characteristics of the in-vitro-generated antibodies, viz., ADCC activity (% PKH26 uptake) against Env C and Env B protein and the killing of Env-reactivated resting memory CD4+ T cells (% p24 reduction and simultaneous % NK cell activation). The percentages of NK cell activation in unstimulated control (background response) were subtracted from the frequencies of NK cell activation after the addition of antibodies to the respective Env-stimulated wells. “R” represents the antibodies generated from R-ADCC LTNPs, i.e., the individuals with plasma ADCC activity, and “NR” represents the antibodies generated from NR-ADCC LTNPs, i.e., the individuals with no plasma ADCC activity. The number corresponds to each LTNP. The median, interquartile range (IQR), and range values are mentioned for both R and NR antibodies.