REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-mNeonGreen	Chromotek	RRID:AB_2827566
Rat anti-BrdU	Abcam	RRID:AB_305426
Mouse anti-UL44 (ICP36)	Virusys	Cat# CA006
Goat anti-Mouse IgG Alexa 555	Invitrogen	RRID:AB_2633276
Goat anti-Mouse IgG Alexa 647	Invitrogen	RRID:AB_2633277
Goat anti-Rabbit IgG Alexa 647	Invitrogen	RRID:AB_2633282
Bacterial and virus strains
HCMV-NeonGreen-UL112	This study	N/A
HCMV-UL32-GFP	Sampaio et al., 2005	N/A
HSV-1 in1374	Preston et al., 1997	N/A
Chemicals, peptides, and recombinant proteins
Fibronectin bovine plasma	Sigma Aldrich	Cat# F1141
Hoechst 33342	Thermo Fisher	Cat# H1399
Polyethylenimine (PEI), branched	Sigma Aldrich	Cat# 408727
Doxycycline (Dox) Hyclate	LKT Labs	Cat# LKT- D5897.5
Phosphonoacetic acid (PAA)	Sigma Aldrich	Cat# 284270
Dulbecco's Modified Eagle's Medium (DMEM) with glucose	Sigma Aldrich	Cat# D5796
Penicillin/streptomycin (100x)	Sigma Aldrich	Cat# P4333
Fetal calf serum (FCS), tetracycline-free	Pan Biotech	Cat# P30-3602
Fetal calf serum (FCS)	Pan Biotech	Cat# P30-0602
1,6-Hexanediol	Sigma Aldrich	Cat# 240117
T4 ligase	New England Biolabs	Cat# M0202L
HisPur Ni-NTA Resin	Thermo Fisher	Cat# 88221
Propylene glycol	Sigma Aldrich	Cat# 398039-25ML
5-Bromo-2′-Deoxyuridine (BrdU)	Sigma Aldrich	Cat# B5002
Experimental models: Cell lines
MRC-5 human embryonic lung fibroblasts	ATCC	RRID:CVCL_0440
Telomerase-immortalized HFF	Bresnahan et al., 2000	N/A
HEK-293T	ATCC	RRID:CVCL_1926
HEK-293A	Invitrogen	RRID:CVCL_0045
T-REx-293	Invitrogen	RRID:CVCL_D585
T-REx-293-mNeonGreen-UL112-113	This study	N/A
Recombinant DNA
pcDNA3.1	Thermo Fisher	Cat# V79020
pcDNA mNeongreen-UL112-113	This study	N/A
pcDNA mNeongreen	This study	N/A
pcDNA His-mNeongreen	This study	N/A
pcDNA M45-mCherry	Muscolino et al., 2020	N/A
pmCherry-Nucleolin	Muscolino et al., 2020	N/A
pEPkan-S2	Addgene	RRID:Addgene_61601
TB40-BAC4	Sinzger et al., 2008	N/A
TB40-BAC4-mNeonGreen-UL112	This study	N/A
pHR-SFFVp-NLS-iLID::EGFP::FTH1	Addgene	RRID:Addgene_122147
pHR-SFFVp-HNRPA1C::mCherry::SspB	Addgene	RRID:Addgene_122668
pHR-SFFVp-mCherry::SspB	This study	N/A
pHR-SFFVp-p43ΔN::mCherry::SspB	This study	N/A
pHR-SFFVp-p84ΔN::mCherry::SspB	This study	N/A
pcDNA6/TR	Invitrogen	Cat# V1025-20
pcDNA4/T0	Invitrogen	Cat# V1020-20
pcDNA4/T0 mNeongreen-UL112-113	This study	N/A
pMD2.G	Addgene	RRID:Addgene_12259
pMDLg/pRRE	Addgene	RRID:Addgene_12251
pRSV-Rev	Addgene	RRID:Addgene_12253
pSG5-p43	Park et al., 2006	N/A
pSG5-p84	Park et al., 2006	N/A
pmCherry-C1	Clontech	Cat# V011976
pcDNA3.1-UL44	Schommartz et al., 2017	N/A
pmCherry-UL112-113	This study	N/A
pcDNA 3.1 UL112-113-mNeonGreen	This study	N/A
pmCherry-UL44	This study	N/A
pmCherry-UL44ΔIDR	This study	N/A
pmCherry-UL44ΔN	This study	N/A
pmCherry-UL44ΔIDR+p43IDR	This study	N/A
Software and algorithms
ImageJ/Fiji	Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, Wisconsin, USA	RRID:SCR_003070
NIS-Elements	Nikon	RRID:SCR_014329
Graphpad Prism 5	GraphPad Software Inc	RRID:SCR_002798
IUpred	Dosztányi et al., 2005	RRID:SCR_014632