FIGURE 2.
Dual culture plate assay for the in vitro inhibition of Colletotrichum acutatum mycelial growth using an antagonistic bacterium GYUN-300: (A) sterile paper discs loaded with bacterial suspensions (108 CFU/mL) placed onto the potato dextrose agar (PDA) plates containing pathogenic fungal spores; and (B) sterile paper discs (6 mm in diameter) loaded with sterilized distilled water (SDW) served as the non-treated control. The inhibition zone was measured 7 days after incubation at 25°C. The experiment was repeated at least once, with each treatment carried out in triplicates (Petri dishes).