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. 2022 Mar 2;13:826827. doi: 10.3389/fmicb.2022.826827

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Copyright © 2022 Kwon, Lee, Kim, Balaraju, Kim and Jeon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of bacterial cell suspensions and culture filtrate (CF) of Bacillus tequilensis GYUN-300 treatment on conidia germination rate (%) of Colletotrichum acutatum and microscopic observation: (A) Conidial germination rate (%) was suppressed by CF and bacterial cell suspensions, while the germination rate (%) was increased in the non-treated control; and (B) microscopic observations of C. acutatum fungal spore germination after GYUN-300 treatment during the incubation period from 8 to 48 h. The germination counting was carried out using a hemocytometer. Bar = 10 μm. The experiment was repeated at least once, with treatments repeated in triplicate producing similar results.