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Construction of recombinant pseudorabies virus (rPRV)-NC56. (A) Schematic diagram of recombinant pseudorabies virus (PRV) construction strategy. (B) Baby hamster Syrian kidney (BHK-21) cells infected with rPRV-NC56 and PRV XJ were observed under fluorescence microscope. (C) PCR identification results of the gE, gI, gB, TK, and partial inserted target genes (ORF5-6) of rPRV-ΔgE/gI-NC-ORF5-6 cultures. (D) PCR identification of the CRISPR/Cas9 deletion of the TK gene.
