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. 2022 Mar 2;13:846079. doi: 10.3389/fmicb.2022.846079

FIGURE 2.

FIGURE 2

Western blot and immunofluorescence assay (IFA) identification of recombinant pseudorabies virus rPRV-NC56. (A) Western blotting analysis of NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) GP5 and M proteins in BHK-21 cells infected with rPRV-NC56. (B) Western blotting analysis of PRV gE and gB protein in BHK-21 cells infected with rPRV-NC56. (C) Detection of the GP5 and M protein expression through an IFA. Green, EGFP; red, GP5 protein-positive cells; blue, DAPI-stained BHK-21 cell nucleus. DAPI, 4′,6-diamidino-2-phenylindole.