FIGURE 4.

Proliferation of C. glabrata in macrophage-like cell. (A) NO production from RAW264.7 cells activated by LPS. The supernatant of cells incubated with LPS and/or NAME was reacted with DAF-FM and then the fluorescence intensity was measured. The fluorescence intensity before the incubation was used as a blank. (B–D) RAW264.7 cells infected with C. glabrata were washed and resuspended in DMEM-B, followed by further incubation for 24 or 48 h. After incubation, macrophage-like cells were lysed and the extracted solution were plated onto YPD with RF solid medium. Colonies formed after further cultivation for a few days at 37°C were counted. Relative cell number using that at 0 h as 1 was shown. The macrophage-like cells were pretreated with LPS (B), LPS and RF (C), or LPS, RF, and NAME (D), respectively, and then further incubated in the presence of these additives. The values are the means and standard deviations of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test.