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. 2022 Mar 2;13:825121. doi: 10.3389/fmicb.2022.825121

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Copyright © 2022 Nasuno, Suzuki, Oiki, Hagiwara and Takagi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection assay using silkworm as a model host organism. (A) Survival rate of silkworm infected with C. glabrata strains. The C. glabrata strains (WT and rib1ΔΔ) were cultured in SC-Ura-His containing RF for 2 days. Resuspended yeast cells were injected to fifth-instar larvae using a needle. The larvae (n = 12) were maintained at 30°C, and survival rate was monitored for 4 days. Statistical significance was examined by Log-rank test using a statistical analysis software BellCurve. The P value for comparison between WT and rib1ΔΔ was lower than 0.01. (B) Survival rate of silkworm infected with C. glabrata strains. The C. glabrata strains (WT and yhb1Δ) were injected to fifth-instar larvae, and the survival rate was monitored for 4 days. There was no statistical significance between WT and ybh1Δ.