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. 2022 Mar 2;10:830432. doi: 10.3389/fcell.2022.830432

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Copyright © 2022 An, Kuo and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of hiPSC-derived brain organoid at Day 27 after culture. Immunostaining of the cryosections obtained from d27 organoids for (A) TUJ1 (a neuron marker)/PAX6 (a NPC marker), (B) Ki67 (a cell proliferating marker)/PAX6, (C) ZO-1 (a tight junction marker)/PAX6, and (D) ARL13B (a ciliary membrane marker)/CENTRIN-3 (a centriole marker). The cilia protruded from the apical surface in 27-day-old brain organoids. Scale bar: 200 μm in (A–D), 10 μm in enlarge (D) (right). White stars in (A) indicate the ventricle-like cavities in the d27 organoids. The enlarged image in [(A), right] is a 90-degree left turn from the left image.