TABLE 3.

Comparison of software tools.

Tool/Feature	References	Language	Skeletonization	Morphological features	NBLAST similarity	Synaptic connectivity
L-Measure	Scorcioni et al., 2008	Java		✓
NBLAST	Costa et al., 2016	R, C++			✓
NeuroM	Palacios et al., 2021	Python		✓
NeuTu	Zhao and Plaza, 2014	C++	✓
TREES toolbox	Cuntz et al., 2010	MATLAB		✓
Vaa3D	Peng et al., 2014	C++	✓	✓
CBLAST	Januszewski et al., 2020	Python, R, C++			✓*	✓
3D BrainCV	Wu et al., 2014	MATLAB	✓	✓
Kimimaro	Silversmith et al., 2021a	Python, C++	✓
RealNeuralNetworks.jl		Julia	✓	✓	✓	✓

*CBLAST uses NBLAST for similarity measure.